#122394 Repro Neuroendocrin Research Assoc.

DESCRIPTION

The UCSD Department of Obstetrics, Gynecology and Reproductive Sciences is composed of 65 faculty and clinical fellows. The department is composed of 6 divisions: Obstetrics and Gynecology, Maternal-Fetal Medicine, Gynecologic Oncology, Reproductive Endocrinology and Infertility, Hospitalist, and Urogynecologic and Reconstructive Surgery.

Under technical supervision, the Repro-Neuroendocrinology Research Associate will perform experiments involving the molecular and cellular analysis of hypothalamic and pituitary cells in a reproductive neuroendocrinology laboratory. Incumbent will help with daily management of the mouse colony, including compliance with ACP and IACUC policies. Assist with analyzing, breeding, and sample mice & conduct surgical procedures. Design and execute studies under the guidance of the supervisor. Assist with performing molecular/cellular biological techniques including RNA/DNA extractions, DNA analysis, cloning, PCR, western blot, running gels, and transcription reactions.

Perform histology techniques such as brain and pituitary tissue collection, tissue cutting (using a cryostat), and preparation of tissue samples for microscopy analysis. Assist in running histological assays such as in situ hybridization and immunocytochemistry. Perform microscopy analyses. The position also involves assisting laboratory personnel with laboratory support, directly training undergraduate researchers or grad students and helping to orient new lab members on lab policies. Other duties include: ensuring laboratory safety, maintaining laboratory inventory, performing general lab maintenance, ordering supplies, and managing radioactivity compliance.

MINIMUM QUALIFICATIONS

• Theoretical knowledge of molecular biology, and previous experience with molecular biology research techniques, such as PCR, DNA/RNA extraction, gel electrophoresis, Western blots, making glycerol stocks, and cloning.

• Theoretical knowledge of animal physiology, endocrinology, and neurobiology.

• Demonstrated experience with cryostate or microtome machine to cut and process neuroendocrine tissues.

• Previous experience and willingness to perform experiments involving the handling, dissection, and sacrifice of laboratory mice, including the collection of blood and tissue.

• Previous knowledge and experience with histological assays in animal tissues, including in situ hybridization and Immunohistochemistry & experience with Grains counting computer program.

• Knowledge of laboratory safety including radioactive/biohazard handling & disposal techniques, & the ability to ensure laboratory compliance with required safety regulations. Previous experience in working with recombinant DNA, biohazardous materials, & toxic chemicals.

• Good interpersonal skills to be effective in communicating & interacting with a variety of people within a large laboratory setting & in administrative components within & outside the University and the Ability to take initiative & handle responsibility in a dependent and efficient manner.

• Experience with sterile technique, preparation of solutions/media, and basic molecular biology techniques.

• Knowledge of rodent anatomy & neuroanatomy, mouse husbandry, and reproductive functions.

• Solid communication skills (verbal and written) in order to interact professionally and effectively with faculty, staff and students.

SPECIAL CONDITIONS

• Employment is subject to a criminal background check.
• Employee must have experience with and be willing to perform experiments involving the handling, dissection, and euthanizing laboratory mice.
• Must work with recombinant DNA, biohazardous research materials, and toxic research chemicals.
• Must be willing to occasionally vary work schedule to accommodate research needs.

Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).

If applicable, life-support certifications (BLS, NRP, ACLS, etc.) must include hands-on practice and in-person skills assessment; online-only certification is not acceptable.

UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!

Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.

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UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html

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UC San Diego Health maintains a marijuana and drug free environment. Employees may be subject to drug screening.